GI-MAP + Zonulin test
Uncover the Hidden Causes of Your Gut Issues with the Most Advanced Microbiome Test Available
The GI-MAP + Zonulin test uses cutting-edge qPCR technology to detect pathogens, inflammation, and leaky gut that standard tests miss – giving you precise, actionable insights for optimal gut health.
Get Your Comprehensive Gut Analysis TodayAre These Symptoms Affecting Your Quality of Life?
Millions of Americans suffer from gut-related issues without knowing the root cause. Traditional testing often misses critical information that could transform your health.
Chronic Digestive Discomfort
Persistent bloating, gas, constipation, or diarrhea that disrupts your daily life.
Unexplained Fatigue
Feeling tired despite adequate sleep, possibly due to hidden gut pathogens.
Brain Fog & Mood Issues
Difficulty concentrating, memory problems, anxiety, or depression linked to gut health.
Autoimmune Conditions
Recurring inflammation, skin issues, or autoimmune symptoms that won't resolve.
The GI-MAP + Zonulin Difference
The GI-MAP (Gastrointestinal Microbial Assay Plus) with Zonulin is a comprehensive stool test that uses cutting-edge quantitative PCR (qPCR) technology to detect and quantify microorganisms in your gut with unprecedented accuracy.
Unlike standard stool tests that rely on culture or microscopy, the GI-MAP analyzes DNA directly, providing truly quantitative results that can detect even low levels of pathogens that other tests miss.
The addition of Zonulin testing provides critical insight into intestinal permeability ("leaky gut"), a key factor in many chronic health conditions.
This advanced testing gives your healthcare provider the precise information needed to create personalized treatment protocols that address the root causes of your symptoms.
Order Your GI-MAP + Zonulin Test
Comprehensive Analysis for Complete Gut Health

Microbial Imbalance Detection
Identifies beneficial bacteria, opportunistic bacteria, and potential pathogens with precise quantification to reveal imbalances in your gut microbiome.
Pathogen Identification
Detects parasites, bacteria, viruses, and fungi that may be contributing to your symptoms, including those often missed by conventional testing.
Inflammation Markers
Measures key markers of intestinal inflammation, helping to identify inflammatory conditions and monitor treatment progress.
Immune Function Assessment
Evaluates immune function markers in the gut, providing insight into how your body is responding to potential threats.
Zonulin for Leaky Gut
Measures zonulin levels, a critical biomarker for intestinal permeability ("leaky gut"), which is associated with numerous chronic health conditions.

Quantitative Results
Provides truly quantitative measurements, allowing for precise treatment planning and the ability to track improvements with follow-up testing.
Success Stories from Real Patients
"After years of being told my symptoms were 'just stress,' the GI-MAP test finally revealed the parasites and bacterial overgrowth causing my problems. Three months after targeted treatment, my symptoms are 90% improved!"
"The GI-MAP + Zonulin test was a game-changer for my autoimmune condition. It revealed significant leaky gut and several infections my previous doctors had missed. Addressing these issues has reduced my inflammation markers dramatically."
"My energy levels have doubled since addressing the issues found on my GI-MAP test. The quantitative results helped my doctor create a targeted protocol that actually worked, unlike the generic approaches I'd tried before."
Who Can Benefit from GI-MAP + Zonulin Testing?
Almost everyone can benefit from a comprehensive gut health assessment, but it's especially valuable for those experiencing:
Digestive Issues
Constipation, diarrhea, bloating, gas, or abdominal pain
Autoimmune Conditions
Hashimoto's, rheumatoid arthritis, psoriasis, or other autoimmune diseases
Mood Disorders
Depression, anxiety, or mood swings linked to gut health
Skin Problems
Acne, eczema, psoriasis, or other chronic skin conditions
Chronic Fatigue
Persistent tiredness or low energy despite adequate rest
Brain Fog
Difficulty concentrating, memory issues, or cognitive problems
Food Sensitivities
Multiple food intolerances or unexplained reactions to foods
Weight Management Issues
Difficulty losing weight despite diet and exercise
Simple Process, Powerful Results
Order Your Test
Purchase your GI-MAP + Zonulin test kit online through our secure portal.
Collect Sample
Follow the simple collection instructions included with your kit in the privacy of your home.
Ship to Lab
Send your sample to our certified laboratory using the pre-paid shipping materials.
Receive Results
Get your comprehensive results within 7-10 business days to share with your healthcare provider.
Frequently Asked Questions
The GI-MAP uses quantitative PCR (qPCR) technology, which provides true quantitative values of microorganisms, not just their presence or absence. This allows for more precise diagnosis and treatment planning. Additionally, the test can detect pathogens at much lower levels than conventional testing methods.
Zonulin is a protein that regulates intestinal permeability. Elevated zonulin levels indicate increased intestinal permeability, commonly known as "leaky gut," which has been linked to autoimmune diseases, food sensitivities, and various chronic health conditions. Testing zonulin provides valuable insight into gut barrier function.
No special preparation is required. However, for the most accurate results, it's recommended to wait at least 4 weeks after taking antibiotics or antifungal medications, and 2 weeks after taking probiotics or digestive enzymes. Detailed instructions are included with your test kit.
Not at all. The kit includes clear, step-by-step instructions and all necessary materials for easy sample collection in the privacy of your home. The process takes just a few minutes and is designed to be as simple and hygienic as possible.
Results are typically available within 7-10 business days after your sample arrives at the laboratory. You'll receive a comprehensive report that can be shared with your healthcare provider to develop a personalized treatment plan.
The GI-MAP + Zonulin test is available through Rupa Health without requiring a doctor's prescription in most states. However, we recommend working with a healthcare provider who can help interpret your results and create an appropriate treatment plan.
How GI-MAP + Zonulin Compares to Other Testing Methods
Feature | GI-MAP + Zonulin | Standard Stool Culture | Basic PCR Tests |
---|---|---|---|
Quantitative Results | |||
Detects Low-Level Infections | |||
Measures Intestinal Permeability | |||
Comprehensive Pathogen Panel | |||
Inflammation Markers | |||
Immune Function Assessment | |||
Antibiotic Resistance Genes |
Transform Your Gut Health with Precision Testing
Stop guessing what's causing your symptoms. Get the comprehensive data you need to address the root causes of your health concerns and start your journey to optimal wellness.
Order Your GI-MAP + Zonulin Test Today